Genetic Transformation in Year 12 Biology

Recently, our Year 12 Biology students successfully completed an exciting practical on genetic transformation. Through this hands-on experiment, students transformed Escherichia coli (E. coli) bacteria with a gene from the bioluminescent jellyfish Aequorea victoria.

The goal was to express the green fluorescent protein (GFP) in the bacteria using plasmids, causing them to glow under UV light. By engaging in this practical, students gained valuable experience in manipulating genetic information and observed the effects of introducing new genetic material into organisms.

This practical not only consolidated their understanding of DNA and RNA, transcription and translation, the cell structures of bacteria, tools and techniques but also showcased the potential of biotechnology in various applications such as the large-scale production of insulin.