Both Year 12 Biology classes have been very busy learning about mutations and a range of Biotechnology tools and different applications. Students put their knowledge of bacterial transformation to the test and completed a practical on pGREEN during Week 9. This practical involved transforming bacteria (E. coli) with a gene from Aequorea victoria; a bioluminescent jellyfish.
The successful transformations of the green fluorescent protein (GFP) of the Aequorea victoria will be expressed in the bacteria; causing them to glow bright green under UV light (as you can see in the image below). Our Year 12 Biologists are practising inquiry skills and learning the impact of errors in experiments which is important for this field of science.
